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Image Search Results
Journal: Journal of Virology
Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
doi: 10.1128/jvi.00529-06
Figure Lengend Snippet: FIG. 1. Leukocytes in efferent lymph and peripheral blood of can- nulated, uninfected sheep 455. Cell counts are plotted in thousands per l as a function of time after cannulation for efferent lymph cells (A) and leukocytes (B) (closed symbols) and mononuclear cells (open symbols) from peripheral blood. Percentages of live-gated, mononu- clear cells staining for the surface markers IgM, CD4, CD8, WC1, and CD14 are plotted for efferent lymph (C) and peripheral blood (D).
Article Snippet: Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200;
Techniques: Staining
Journal: Journal of Virology
Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
doi: 10.1128/jvi.00529-06
Figure Lengend Snippet: FIG. 5. Cell lineages and blasts among efferent lymph cells of mock-infected and infected sheep. (A) Percentages of live cell labeling on the surface for IgM, CD4, CD8, WC1, and CD14 are plotted for each animal relative to the day of injection with allogeneic cells. Horizontal reference lines represent the average percentage for a given lineage as measured in each animal before injection of allogeneic cells. (B) Reciprocal oscillation of IgM and CD8 lymph cells in infected, cannulated sheep. (C) Percentages of blasts among lymph cells of different lineages. Blasts were quantified as percentages of lymph cells staining for the indicated surface marker by gating on cells with high forward and side scatter (infected animals, closed symbols; mock-infected animals, open symbols). Averages for infected (solid lines) and mock-infected (dotted lines) animals were calculated using measurements made either on the same day or on adjacent days after injection of allogeneic cells.
Article Snippet: Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200;
Techniques: Infection, Labeling, Injection, Staining, Marker
Journal: Journal of Virology
Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
doi: 10.1128/jvi.00529-06
Figure Lengend Snippet: FIG. 8. Percentages of BrdU cells present among efferent lymph cells and PBMCs after intravenous pulses of BrdU. The percentage of BrdU-stained cells, as determined by flow cytometry, is plotted for the total live-cell population as well as within IgM, CD4, CD8, and WC1
Article Snippet: Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200;
Techniques: Staining, Cytometry
Journal: Journal of Virology
Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
doi: 10.1128/jvi.00529-06
Figure Lengend Snippet: FIG. 1. Leukocytes in efferent lymph and peripheral blood of can- nulated, uninfected sheep 455. Cell counts are plotted in thousands per l as a function of time after cannulation for efferent lymph cells (A) and leukocytes (B) (closed symbols) and mononuclear cells (open symbols) from peripheral blood. Percentages of live-gated, mononu- clear cells staining for the surface markers IgM, CD4, CD8, WC1, and CD14 are plotted for efferent lymph (C) and peripheral blood (D).
Article Snippet: For double labeling,
Techniques: Staining
Journal: Journal of Virology
Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
doi: 10.1128/jvi.00529-06
Figure Lengend Snippet: FIG. 5. Cell lineages and blasts among efferent lymph cells of mock-infected and infected sheep. (A) Percentages of live cell labeling on the surface for IgM, CD4, CD8, WC1, and CD14 are plotted for each animal relative to the day of injection with allogeneic cells. Horizontal reference lines represent the average percentage for a given lineage as measured in each animal before injection of allogeneic cells. (B) Reciprocal oscillation of IgM and CD8 lymph cells in infected, cannulated sheep. (C) Percentages of blasts among lymph cells of different lineages. Blasts were quantified as percentages of lymph cells staining for the indicated surface marker by gating on cells with high forward and side scatter (infected animals, closed symbols; mock-infected animals, open symbols). Averages for infected (solid lines) and mock-infected (dotted lines) animals were calculated using measurements made either on the same day or on adjacent days after injection of allogeneic cells.
Article Snippet: For double labeling,
Techniques: Infection, Labeling, Injection, Staining, Marker
Journal: Journal of Virology
Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
doi: 10.1128/jvi.00529-06
Figure Lengend Snippet: FIG. 1. Leukocytes in efferent lymph and peripheral blood of can- nulated, uninfected sheep 455. Cell counts are plotted in thousands per l as a function of time after cannulation for efferent lymph cells (A) and leukocytes (B) (closed symbols) and mononuclear cells (open symbols) from peripheral blood. Percentages of live-gated, mononu- clear cells staining for the surface markers IgM, CD4, CD8, WC1, and CD14 are plotted for efferent lymph (C) and peripheral blood (D).
Article Snippet: Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200; mouse-anti-bovine CD4 (clone GC1A, IgG2a isotype; cross-reactive with sheep; Veterinary Medical Research and Development, Inc.) used at 1:50; mouse-anti-bovine CD8 (clone CC63, IgG2a isotype; cross-reactive with sheep;
Techniques: Staining
Journal: Journal of Virology
Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
doi: 10.1128/jvi.00529-06
Figure Lengend Snippet: FIG. 5. Cell lineages and blasts among efferent lymph cells of mock-infected and infected sheep. (A) Percentages of live cell labeling on the surface for IgM, CD4, CD8, WC1, and CD14 are plotted for each animal relative to the day of injection with allogeneic cells. Horizontal reference lines represent the average percentage for a given lineage as measured in each animal before injection of allogeneic cells. (B) Reciprocal oscillation of IgM and CD8 lymph cells in infected, cannulated sheep. (C) Percentages of blasts among lymph cells of different lineages. Blasts were quantified as percentages of lymph cells staining for the indicated surface marker by gating on cells with high forward and side scatter (infected animals, closed symbols; mock-infected animals, open symbols). Averages for infected (solid lines) and mock-infected (dotted lines) animals were calculated using measurements made either on the same day or on adjacent days after injection of allogeneic cells.
Article Snippet: Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200; mouse-anti-bovine CD4 (clone GC1A, IgG2a isotype; cross-reactive with sheep; Veterinary Medical Research and Development, Inc.) used at 1:50; mouse-anti-bovine CD8 (clone CC63, IgG2a isotype; cross-reactive with sheep;
Techniques: Infection, Labeling, Injection, Staining, Marker
Journal: Journal of Virology
Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
doi: 10.1128/jvi.00529-06
Figure Lengend Snippet: FIG. 8. Percentages of BrdU cells present among efferent lymph cells and PBMCs after intravenous pulses of BrdU. The percentage of BrdU-stained cells, as determined by flow cytometry, is plotted for the total live-cell population as well as within IgM, CD4, CD8, and WC1
Article Snippet: Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200; mouse-anti-bovine CD4 (clone GC1A, IgG2a isotype; cross-reactive with sheep; Veterinary Medical Research and Development, Inc.) used at 1:50; mouse-anti-bovine CD8 (clone CC63, IgG2a isotype; cross-reactive with sheep;
Techniques: Staining, Cytometry